Engineers at Massachusetts Institute of Technology have developed carbon nanotubes into sensors for cancer drugs and other DNA-damaging agents inside living cells.
The sensors, made of carbon nanotubes wrapped in DNA, can detect chemotherapy drugs such as cisplatin as well as environmental toxins and free radicals that damage DNA.
"We've made a sensor that can be placed in living cells, healthy or malignant, and actually detect several different classes of molecules that damage DNA," said Michael Strano, senior author of a paper on the work appearing in the Dec. 14 on-line edition of Nature Nanotechnology.
Such sensors could be used to monitor chemotherapy patients to ensure the drugs are effectively battling tumors. Many chemotherapy drugs are very powerful DNA disruptors and can cause serious side effects, so it is important to make sure that the drugs are reaching their intended targets.
The sensor can detect DNA-alkylating agents, a class that includes cisplatin, and oxidizing agents such as hydrogen peroxide and hydroxyl radicals.
Using the sensors, researchers can monitor living cells over an extended period of time. The sensor can pinpoint the exact location of molecules inside cells, and for one agent, hydrogen peroxide, it can detect a single molecule.
The new technology takes advantage of the fact that carbon nanotubes fluoresce in near-infrared light. Human tissue does not, which makes it easier to see the nanotubes light up.
Because they are coated in DNA, these nanotube sensors are safe for injection in living cells. In future studies, the researchers plan to use the sensors to study the effects of various antioxidants, such as the compounds in green tea, and learn how to more effectively use toxic chemotherapy drugs.
(Xinhua News Agency December 15, 2008)
